In this webinar, Dr. Erwin Schoof will describe how a 384-well workflow, using standard off-the-shelf components, allows characterization of single cells to a protein depth of well over 1,000 proteins per cell. Dr. Schoof will introduce the latest generation HRAM Thermo Scientific™ Tribrid™ MS instrumentation with FAIMS technology for single cell-level sensitivity. When combined with new Thermo Scientific™ TMTPro reagents and optimized chromatography, researchers can analyze >100 single cells per day per instrument. An in-house developed computational pipeline, SCeptre, helps derive biological meaning from the data.
These advances in single cell MS sample preparation and analysis provide a robust way to collect cellular proteomes from mammalian cells, paving the way for large-scale single cell MS experiments at unprecedented depth.
What you will learn:
- How to use standard consumables to conduct high-throughput single cell proteomics
- How RNA-seq inspired tools can help the single cell MS community
- How to optimize instrument and chromatography parameters to boost proteome coverage and cell throughput
